The study investigated the effect of B. cereus probiotic on the growth performance in T. Trichopterus. B. cereus strain isolated from raw milk was characterized by means of standard biochemical and 16S rRNA gene sequencing studies. Fish feed with and without bacteriocin was prepared and it was fed to the juvenile fishes. After 60 days trial, specific growth rate was increased with the addition of Bacteriocin in fish diets. Likewise the survival rate of juvenile T. trichopterus was 50% for control and 94% the fish diet supplemented with the bacteriocin produced by B. cereus. Thus it was found that in addition of being effective bacteriocin producing B. cereus could also promote the growth of the fish effectively and thus it play an important role in aquaculture nutrition.