Chronic Suppurative Otitis Media (CSOM) is a chronic inflammation of the middle ear and mastoid cavity, which presents with recurrent ear discharge or otorrhea through a tympanic membrane perforation. If left untreated, it can lead to serious intracranial & extracranial complications. Hence, the present study was undertaken to identify the pattern of aerobic microorganisms (bacterial and fungal) causing CSOM & to determine the antibiotic sensitivity pattern of the aerobic bacterial isolates prevalent in our hospital. The study included 130 clinically diagnosed cases of CSOM. Samples collected from the patients were processed using standard microbiological techniques. Out of 130 clinically diagnosed cases of CSOM, 116 samples were culture positive. The most predominant organism causing CSOM among aerobic bacteria was Pseudomonas aeruginosa, followed by Staphylococcus aureus. Of the fungal isolates Aspergillus niger was commonest followed by Candida albicans & Aspergillus flavus. Amikacin, gentamicin and ciprofloxacin were effective against most of the gram negative bacilli. Gentamicin, doxycycline & chloramphenicol were effective against gram positive cocci. The Knowledge of microbial pattern prevalent in our locality and their antibiotic sensitivity pattern helps in rational use of antibiotics and thus prevent the emergence of resistant strains and also the complications associated with CSOM.