eng Excellent Publishers International Journal of Current Microbiology and Applied Sciences (IJCMAS) CODEN(USA)-IJCMO9 2319-7692 2319-7706 2015-12-20 4 12 712 717 article Solid Substrate Fermentation using Agro Industrial Waste: New Approach for Amylase Production by Bacillus licheniformis Palki Sahib Kaur 1 Sukhjeet Kaur 2 Hardish Kaur 3 Arpit Sharma 4 Pushap Raj 5 Shaily Panwar 6 Department of Biotechnology, CGC, Landran, Mohali, I.K.G.Punjab Technical University, Kapurthala, India Department of Biotechnology, SUSCET, Tangori, India Department of Biotechnology, Government College for Girls Sec. 42, Chandigarh, India

In the present study amylase production was optimized using Solid Substrate fermentation after screening of four types of agro industrial waste material as substrate i.e wheat straw, paddy straw, sugarcane waste, and maize straw. The results obtained in the present study indicated Bacillus licheniformis as a potential strain for a-amylase production using solid-state fermentation with paddy as substrate. Various physiochemical parameters were also optimized like fermentation period, pH and inoculum concentration. Maximum enzyme production was found to be 37ºC and pH7. Effect of inorganic salt (NaCl, FeCl , MgSO , KH PO and CaCl ) and various surfactants (Tween20, Tween80, PEG,

 http://ijcmas.com/vol-4-12/Palki Sahib Kaur, et al.pdf Amylase Optimization Agro industrial Solid substrate fermentation