Enteric bacteria are normal inhabitants of the intestines of humans and other animals. Sewage contains high numbers of potentially very pathogenic enteric bacteria known as fecal coliforms In their natural habitat enteric bacteria are typically harmless but they can produce severe disease symptoms when ingested by susceptible individuals, Bacteriophages are viruses that infect bacterial cells. Phages are nonliving agents and thus require the use of the host s metabolic processes to replicate itself. In this study, the phages of interest are those that infect and lyses E. coli host cells. When phages are released from the ruptured host, distinct zones of clearing (plaques) form. The original E. coli host cells for this experiment came from a sample of raw sewage. In order to obtain the bacteriophage, a procedure of enrichment, isolation, dilution and seeding was followed, the presence of distinct plaques indicated that lytic bacteriophage had been successfully amplified, separated and grown. This study included determination of phage titre. Phage titer is determined by serial dilution of phage filterate (10-1, 10-2, 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, and 10-9) the dilution factor gave the best countable number of plaques is10-6. This dilution factor was then used for further characterization. The objectives of this study were to isolate phage from sewage sludge, identify its enteric bacterial host, and to determine the Phage titer.