Mosquito midgut microbiota plays a crucial role in its vector susceptibility and pathogenic or parasitic interaction. To understand the bacterial diversity in the midgut of Aedes albopictus mosquitoes, we conducted a screening study of the gut microbes of these mosquitoes which were collected from fields and reared in the laboratory. Mosquitoes are haematophagous vector and carries parasites and pathogens of numerous diseases like Dengue, Filaria, Malaria, etc. Mosquitoes can be considered as holobiont units in which host (mosquitoes) and its gut microbiota are involved in a complex reciprocal interaction. The naturally acquired microbiota can modulate mosquitoes’ vectorial capacity by inhibiting the development of pathogens and parasites. But enough care has not been taken in West Bengal to study on midgut microbiota of Aedes mosquitoes. Therefore a preliminary attempt has been undertaken to study the mid gut microbiota of Aedes (Aedes albopictus) mosquitoes collected from Diamond Harbour (South 24 Parganas) areas of west Bengal.