The Extended Spectrum -Lactamase (ESBL) producing bacteria are increasingly causing urinary tract infections (UTIs) both in hospitalized and outpatients. Despite the widespread availability of antimicrobial agents UTI has become difficult to treat empirically because of appearance of pathogens with increasing resistance to antimicrobial agents. To determine the ESBL producing gram negative uropathogens and their antibiotic resistance pattern. This study was conducted in the department of microbiology, RajaRajeswari Medical College & Hospital, Bengaluru, over a period of 1 year. During this period around 5039 urine samples were received. Standard microbiological techniques were used to isolate and identify the organisms and to determine the antibiotic resistance pattern. A total of 793 aerobic bacterial isolates were obtained from 5039 urine samples, which comprised of 689 GNB. E. coli was the most predominant bacteria 65.93% followed by Klebsiella pneumoniae 15.41%. 28.63% (195/681) of GNB were ESBL producers. Predominant ESBL producers were E. coli 35.18% followed by Citrobacter spp. 30%, K. pneumoniae 21.90%. ESBL production was more prevalent in E. coli followed by K. pneumoniae. Screening and monitoring of ESBL production and antimicrobial susceptibility testing are necessary to avoid treatment failure in patients with UTI.