The field experiment during spring season 2013 in plastic house in the research and studies division, Horticulture Department, Ministry of Agriculture. The field experiment was carried out to investigate the effect of foliar sprays with the sane nitrogen levels on the production of micro potato tubers in vitro of potatoes (Lizita cultivar).The sprays was carried out twice the first at the end of vegetative growth stage and the scand at tuber initiation stage. The experimental results showed that the levels (N4) gave the significantly the highest number of tuber, yield per plant, highest of plant dry weight of vegetative parts, number of leaves, the percentage of starch, the percentage of dry matter, the percentage of protein 6.73 / plant, 34.01 gm, 23.00 cm, 1.26 gm. 12.73 leaf / plant 13.4%,18.6%,16.9% respectively, while the levels ( N2) gave the highest of tuber diameter, leave area number of stem (5.43 mm, 333.6 cm2 12.17 stem plant ) respectively.