Lysinibacillus sphaericus SNCh5 is a gram positive rod shaped and mesophilic bacterium. In the present study we investigated that L.sphaericus SNCh5 has important properties as it solubilised 10g/L of Ca3PO4 and pH value of SNCh5 inoculating broth was decreased to 2.18. It also produced maximum 142.7 μg/mL ammonia and 56.8 μg/mL of IAA. About 87.4% of in vitro BNF was also performed in 4 days and 89.9% after utilization of 8% sucrose in nitrogen free broth medium. The effect of SNCh5 on Trigonella feonum-graecum (methi) and Vigna radiata (mung beans) seeds after treatment was observed. Growth parameters under natural condition in the pot experiment were also estimated in SNCh5 treated Vigna radiate seeds. Genome sequence of SNCh5 bacteria was matched with Lysinibacllius sphaericus and the sequences were submitted to NCBI (Accession No. KT380157).