Occupational Exposure refers to exposure to potential blood-borne infections that may occur in healthcare settings during performance of job duties. World Health Organization estimates that 3 million percutaneous exposures occur annually among 35 million workers (HCW) globally, with over 90% occurring in resourceconstrained countries. In addition, such exposures can cause tremendous anxiety, fear and stress among HCW that can have a negative impact on the HCW while providing their services. Universal safety precautions deals with measures to prevent exposure of health care workers to source of infection. While post exposure prophylaxis deals with prophylactic measures taken to prevent establishment of infection after occupational/accidental exposure of health care worker/provider to infectious source material has occurred.Present study aimed to examine and study the knowledge, attitude and practice of post exposure prophylaxis on occupational/accidental exposure among health care workers/providers at a tertiary care hospital in western Uttar Pradesh. 240 HCW volunteers were divided in groups of 10-20 in number and questionnaire were distributed. Answers revealed , 23(9.5%) volunteers had undergone training regarding PEP. Majority were unaware of PEP, thus had casual/careless attitude. 56(23.33%) volunteers gave history of occupational/accidental exposure. In 0(0%) cases of occupational/accidental exposure source code & exposure code was initiated. neither were brought in notice of hospital superintendent and thus as a result neither any record of HCW occupational/accidental exposure in hospital recorded and maintained. 2(3.5%) volunteers with occupational/accidental exposure took PEP on their own that too after 48 hours. Training directed towards proper knowledge and adherence to USP and PEP can bring about a positive change resulting in willingness of HCW to provide care for patients with HIV infection and other blood borne pathogens.