Trichomonas vaginalis is a flagellated parasite, causes sexually transmitted infections mainly in women. It is usually found with other sexually transmitted infections such as Chlamydia, Gonorrhoea, Syphilis and Herpes simplex virus type II and is a sensitive marker of highly sexual behaviour. It causes pelvic inflammatory disease and adverse pregnancy outcomes. Hence this study is carried out to know the prevalence of Trichomonas vaginalis infection by various laboratory diagnostic tests. A cross sectional study was conducted on 135 women complaining of vaginal discharge. Vaginal swabs were collected and examined microscopically by wet mount, culture and Pap smear examination. Out of 135 patients Trichomonas vaginalis was identified in 26(19.25%) by wet mount and Pap smear examination. By culture Trichomonas was identified in 30 cases (22.22%). Majority of the women were with infection was in the age group of 31- 35 years. Trichomonas infections were seen in 22% of women in reproductive age group. Culture was the gold standard method than wet mount examination. Pap smear examination was also equally effective as wet mount. As Trichomonas infection indicates other sexually transmitted infections so is better to test other infections also in reproductive age.