The present study has been designed to investigate the prevalence and risk factors of RTIs in HIV positive/negative women in India population. As, Infection with HIV causes immune suppression of the host, which make them more susceptible for various RTIs. The study included 120 HIV seropositive and 100 seronegative women. Participants were interviewed regarding complaints and risk factors. Cervical scrapes samples were tested for Human papillomavirus(HPV), Chlamydia trachomatis(CT), Trichomonas vaginalis(TV) and Neisseria gonorrhoeae(NG) infection by using PCR and Bacterial vaginosis(BV) was detected through cervical cytology. Among the HIV-seropositive cases, positivity for the infections including HPV 19%(23/120), CT 12%(14/120), TV 6%(7/120) and BV 21%(25/120) was observed. In control subjects, the prevalence of infections was HPV 4% (4/100), CT 2%(2/100), TV 2%(2/100) and BV 4%(4/100) was observed. Multiple partners (p=0.0005), use of oral contraceptives (p=0.0011) and smoking habits (p=0.0164) were found to be positively associated as risk factors for RTIs. NG infection was not observed in any sample. The use of oral contraceptives and smoking habits may be a risk factor for RTIs in HIV infected women in India. Hence, there is a need to continuously screen, counsel, treat and monitor the trends of RTIs.