To assess the feasibility of detecting Rubella virus (RV), Human cytomegalovirus (HCMV) and Herpes simplex virus (HSV) in fetal samples with antenatal suspicion of congenital infection and to correlate the results with ultrasonographic findings. A prospective study was carried out using amniotic fluid / cord blood samples obtained from pregnant women with fetuses suspected as possibly having an intrauterine congenital infection ,. Samples were processed by PCR for the presence of RV, HCMV, and HSV. RV amplified products were further sequenced to identify the genotype. Out of 44 samples tested, 37 were included in this study for analysis as remaining 7 had an abnormal karyotype. A total of 6 samples tested positive for viral PCR (2 CMV and 4 RV; 5 AF and 1 FBS). The commonest indication for fetal testing for viral PCR was echogenic small b owel, followed closely by intrauterine growth restriction and non-immune hydrops. Approximately half of the cases had more than one ultrasound marker at presentation. None of the samples were positive for HSV. It is feasible to test fetal samples for CMV, RV and HSV in India. No ultrasound marker appeared to be specific for a particular viral infection.