Human Cytomegalovirus (CMV) is one of the most significant pathogens infecting immunosuppressed individuals. CMV is known to be a significant cause of morbidity and mortality following blood transfusion in immunocompromised individuals. The immunosuppressed population for whom CMV free blood products are requested is increasing due to advances in medical care. The most effective way to minimize the risk of CMV transmission in high risk recipients would be to administer CMV free blood products. The study was performed to find out the seroprevalence of Human CMV among voluntary blood donors in Tirunelveli.A total of 92 voluntary blood donors were tested for IgM and IgG anti- CMV antibodies by ELISA technique .Demographic details and laboratory results were analyzed. Among the 92 donors, 82 (89%) were males and 10 (11%) were females. It was found that 7 (7.6%) donors were positive for IgM and 76 (82.6%) donors were positive for IgG.Human CMV is highly prevalent and is a threat to the safety of blood transfusion. Hence it would be useful to screen blood donors for CMV to identify the CMV seronegative donors to maintain an inventory and use them as donors for immune compromised patients .Other preventive strategies like leukoreduction or pathogen inactivation can be made available to prevent CMV transmission.