This study was carried out to observe the frequency of Hepatitis B,Hiv,and syphilis and the value of routine prenatal screening among the pregnant women, attending antenatal clinic of Bhaskar general hospital at Ranga Reddy dist. A serological screening was carried out during the period January 2015 to October 2015. All the pregnant women who attended the clinics were screened for Syphilis using Rapid Plasma Reagin (RPR) test. All those who were positive were confirmed using Treponema pallidum haemagglutination (TPHA) test. Patients were screened for Hepatitis B by Immuno Chromatographic Technique (ICT) device designed for qualitative detection of HBsAg in serum. Those found positive on screening test were confirmed by ELISA. Finally, samples were tested for antibodies to HIV by three different methods as per Strategy III of the National AIDS Control Organization by using different systems of testing to establish a diagnosis of HIV. Seropositivity of hepatitis B was 0.86%, syphilis was 0%, and HIV was 0.97%. Testing for human immunodeficiency virus (HIV), syphilis, and hepatitis B surface antigen (HBsAg) was done to assess the extent of seropositivity in pregnant women and to re-evaluate the need for routine antenatal care screening.