Kaziranga National Park (KNP) is a World Heritage site and one of the largest tracts of protected land in the sub-Himalayan belt. Though various studies have been done on tree and herbaceous plants, micro-floral diversity in KNP is yet to be explored. The present investigation is an attempt to record algal diversity from aquatic habitat in two Range areas following proper methodology of collection of samples and identification procedures. All together 91 species of algae are found belongs to the family Chlorophyceae, Bacillariophyceae and Cyanophyceae among which 89 algae species are new report from this protected area. The result of this study gives information about algal flora that may be added to the list of plants in KNP.