Urinary tract infections (UTIs) can be complicated if not diagnosed early and treated. Bacteriological analysis of urine samples collected from female students staying in female hostel of Chukwuemeka Odumegwu Ojukwu University, Uli Campus was carried out. A total of 100 urine samples were collected from the students, classified and analyzed for urinary tract infection (UTI) using pour plate method. The bacterial organisms isolated from the urine samples were characterized and identified using their colony descriptions, morphological and biochemical characteristics. The isolates were subjected to sensitivity test against conventional antibiotics using disc diffusion method. The bacteria isolated from the urine samples include Escherichia coli 53(56.38%), Staphylococcus aureus 11(11.70%), Staphylococcus saprophyticus19(20.21%), Proteus mirabilis 4(4.26%) and Klebsiella pneumoniae 7(7.45%). The results of the study revealed that 29.00% of the samples analyzed were positive for UTI (105 CFU/ml), 34.00% suspected for UTI (102-104 CFU/ml) and 37.00% were normal (below 102 CFU/ml). Significant UTI was seen most among students of age 20-24 years (37.88%), single females (30.85%) and students studying non medical related courses (48.48%). Most of the conventional antibiotics were active against the isolates, of which ciprofloxacin showed the highest inhibition zone diameter (18-26mm) against Gram positive and Gram negative isolates. However, the two Gram positive bacteria (Staphylococcus aureus and Staphylococcus saprophyticus were resistant to bacitracin, vancomycin, levofloxacin, amikacin and azithromycin. From this study, periodic testing of UTI and also sensitivity testing prior to treatment is advocated. Those found to be infected need to be treated with antibiotics like ciprofloxacin to avoid complications.