To isolate and identify various dermatophytes from clinically suspected cases of dermatophytoses. Clinical specimens from various suspected cases of dermatophytosis attending the out-patient department of Dermatology, Maharishi Markandeshwar Medical College and Hospital, Solan were studied in Microbiology Department over a period of one year. Skin scrapings, nail clippings, hair stubs were collected from patients by standard procedures. Samples were processed by 10-40% potassium hydroxide mount, culture was done on Sabouraud dextrose agar with chloramphenicol and actidione and on dermatophyte test medium. Seventy cases (58.3%) were positive for fungus in direct microscopy while forty eight (40%) were culture positive. Trichophyton mentagrophytes 24 (50%) was found to be the commonest isolate followed by Trichophyton rubrum 15 (31.3%), Epidermophyton floccosum 4 (8.3%), Trichophyton tonsurans 2 (4.2%) and Microsporum species 3 (6.2%). Trichophyton mentagrophytes was the commonest isolate. Mycological examination aids in the diagnosis of various dermatophytoses for their effective management.