Neonatal sepsis is one of the commonest causes of neonatal mortality in the developing world. To determine the bacteriological profile of neonatal septicaemia, their antibacterial susceptibility pattern (AST) Blood culture specimens were collected from 100 neonates. Identification of organisms, their AST was done. Gram negative bacteria were more frequently isolated than gram positive bacteria. The gram positive bacteria were highly resistant to penicillin. Gram negative bacteria also exhibited high resistance to the commonly prescribed group of drugs such as penicillins, cephalosporins and aminoglycosides. ESBL production was seen in 52.9% of Klebsiella and 50% of E.coli, Klebsiella.pneumoniae was the most common bacteria associated with neonatal sepsis. Gram negative bacteria were isolated predominantly and many of them were resistant to several groups of drugs. Also high resistance was seen to third generation cephalosporins in case of Klebsiella pneumoniae and E.coli due to ESBL production.