The main objective of this research was the optimization of some culture conditions to enhance Alpha amylase production. Screening, isolation and identification of - amylase producing bacteria from different locations in Khartoum was determined. Twenty five soil samples were collected from different locations. The bacterial isolates for amylase production reflected by a large zone using iodine method. These isolates were identified according to microscopical and biochemical test. 28%were found to be Bacillus subtilis while 24% was found to be Bacillus licheniformis Statistical experimental design was used to optimize the physical conditions such as temperature and incubation time to enhance alpha amylase production from Bacillus licheniformis. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular alpha amylase was achieved at 0.445 mgml/hr when the temperature and time of incubation were 47°C and 46.3 hr. respectively.