To study the occurrence and causative agents (fungus) of dermatophytosis in patients attending Dermatology and Venereology outpatient department of HIMS hospital, Dehradun, Uttarakhand India. A total of 124 samples were collected including infected skin, hair and nail samples for a period of 1 year. Before collecting the samples, 70% alcohol was applied to the infected area and ensured that it was totally dry. Skin samples were collected by scrapping, nail samples by clipping and hair samples collected by using sterile scalpel or forceps. Identification of the causative pathogen was done by performing slide culture, lacto-phenol cotton blue mount, hair perforation tests and urease tests Dermatophytosis was manifested clinically most common in the age group 21-30 years (24.8%) followed by 31-40 years (23.9%). Amongst the various clinical patterns, Tinea corporis was the commonest (49.2%) type followed by Pityriasis versicolor (12.1%), Tinea unguium (12.1%) and Tinea pedis (12.1%). Out of 109 clinically suspected cases of Dermatophytoses, fungi was demonstrated in 60 cases (55%) either by KOH mount and/or culture. Trichophyton rubrum was the commonest (37.5%) species isolated followed by Trichophyton mentagrophytes (20.8%). Further intensive epidemiological studies of dermatophytic fungus-induced dermatophytosis, which have public health significance, are needed.