Candidiasis is the commonest vaginal infection affecting about 75% women during reproductive age group. Candida albicans is the most common species causing Candidiasis but there has been an increase in Candida non albicans as reported in various studies. Isolation and speciation of Candida from cases of vulvovaginitis and their antifungal susceptibility. Materials and methods: This study group includes 100 high vaginal swabs from patients with vulvovaginitis attending OPD, KGH, Visakhapatnam. Standard mycological tests for candida isolation , speciation and antifungal susceptibility were done. Out of 100 samples,90 were culture positive for Candida. The most commonly isolated species was C.parapsilosis (33.3%) followed by C.albicans (22.2%), C.glabrata (20%), C.dubliniensis (14.4%), C.krusei (6.6%) and C.tropicalis (3.3%). Most of the Candida species were sensitive to Amphotericin- B, Ketoconazole, Clotrimazole and nystatin and were relatively resistant to Fluconazole and Itraconazole. In this study, there is an increase in infections with Candida non-albicans species and emergence of azole resistant Candida albicans and non albicans species specify the need of species identification and antifungal susceptibility. Antifungal susceptibility of Candida species prior to initiation of therapy is necessary to prevent the emergence and spread of drug resistance.