Many viruses can produce infectious damage to the Liver. EBV and CMV occasionally cause symptomatic hepatitis, as part of the mononucleosis syndrome. The primary hepatitis viruses are diverse group. The alphebit soup of hepatitis is summarised below. Several other viruses have been described as possible agents of hepatitis e.g. G.Virus (or G B variant of Hepatitis C virus, a member of the family Flaviviridae, as well as TT virus and SEN virus, both are the members of the family Circoviridae). Here prevalence of HCV and CHC (Chronic Hepatitis C) are increasing due to aging of the infected population and progression of liver fibrosis. History of repeated blood transfusions in a case of abortions in females, major trauma, alcoholism, biliary stenosis, parasitic infections and secondary liver infections, jaundice and needle stick injuries are chosen for study. Recent reports show that extra hepatic immunologic manifestation, such as cryoglobulinemia and rheumatoid factors are a prominent part of Hepatitis C infections and they are for Hepatitis B also. Seventy five patients are screened for various diseases. All serum samples collected and stored at -20°C freezer. ELISA was conducted with positive and negative controls. All the serum samples collected from date 1st October 2015 to 30th October 2015 at Sivagiri Sree Narayana Medical Mission Hospital, Varkala, Trivandrum after obtaining permission from the authorities. Out of 75 patient s sample, 24 males and 51 females and most of them are middle age females groups having HCV infection.