International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 49-56
Effect of process parameters on the enzyme activity of a novel Beauveria bassiana isolate
Sapna Mishra, Peeyush Kumar and Anushree Malik*
Applied Microbiology laboratory, Centre for Rural Development & Technology, Indian Institute of Technology Delhi, New Delhi- 110 016, India *Corresponding author e-mail:
Enzyme production potential of a native Beauveria bassiana (HQ917687) isolate was elucidated through the assessment of effect of incubation period and temperature. Evaluation of enzyme activity of fungal filtrate showed increase in activity with increase in incubation time. Maximum enzyme activity was observed on 5 th day [chitinase (5.24 U/ml), Pr1 protease (4.66 U/ml), lipase (2.26 U/ml)] or 4 th day [pr2 protease (5.48 U/ml)] of the culture growth. The enzymes showed variation in activity with temperature, with maximum chitinase (5.05 U/ml) and pr1 (4.31 U/ml) activity at 40 ºC, whereas maximum activity of pr2 was observed at 50 ºC (4.52 U/ml). The optimum activity of enzyme was observed between pH 5-7, while beyond this range decrease in enzyme activity was observed with increase in medium acidity or alkalinity. The results obtained from this study are expected to help in experimental design and biotechnological modification using particular strain of B. bassiana.
Beauveria bassiana; enzyme; incubation period; temperature; activity