| International Journal of Current Microbiology and Applied Sciences |
| ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 316-327 |
| The role of black soybean tempe in increasing antioxidant enzyme activity and human lymphocyte proliferation in vivo |
| Nurrahman1 , Mary Astuti2 , Suparmo2 dan Marsetyawan HNE Soesatyo3 |
| 1Departement of Food Technology, Semarang Muhammadiyah University, Indonesia 2Departement of Food Science, Gadjah Mada University, Yogyakarta, Indonesia 3Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia *Corresponding author e-mail: nurrahmanmail@yahoo.com |
| ABSTRACT |
| The protective effect of flaxseed oil (FSO) and vitamin E (Vit E) on potassium bromate (KBrO3)-induced oxidative stress and hepatonephrotoxicity in male rats was investigated. Forty five mature male rats were randomly distributed into 5 groups (n=9). Group (1) was negative control and the other 4 groups were injected with a single dose of KBrO3 (125 mg/kg, i.p.) to induce oxidative stress. Group (2) was used as a positive control, while groups (3), (4) and (5) were orally given FSO (1 ml/rat, 500 mg/kg), Vit E (0.5 ml/rat, 50 mg/kg) and FSO and Vit E in combination, by the same doses daily for 8 weeks respectively. Blood samples were collected for separating the serum for assessment of hepatorenal function. Livers and kidneys were taken for preparing tissue homogenates for biochemical analysis. The results showed that oxidative stress induced by KBrO3 in rats caused significant decreases in body weight gain and feedIn some hospitals, soybean porridge is used by nutritionists for children who have diarrhea therapy. This is likely related to the recovery of the immune system, both systemically and in the digestive tract. Rats, fed with black soybean tempe diet for 35 days, had higher T cell proliferation index than standard diet rats. This study aims to determine the potential of black soybean tempe in modulating antioxidant enzyme activity and proliferation of human lymphocytes in vivo. A total of 21 respondents were divided into three groups, namely placebo consumption group, tempe extract capsules consumption and tempe consumption group. Interventions were conducted for 28 days in respondents resident. During the intervention all respondents only consumed provided food by researchers. Each respondent in the treatment group consumed 100 grams tempe per day, while those in soybean extract group consumed 3 capsules. Black soybean tempe consumption in humans could enhance the immune system by increasing T cell proliferation and lymphocytes resistance to hydrogen peroxide. Consumption of black soybean tempe tends to enhance B cell proliferation, enzyme activity of SOD, catalase and glutathione peroxides. It can be concluded that consuming tempe could enhance the immune system, especially the cellular immune system. |
| Keywords |
| Black soybean tempe; antioxidant enzyme; lymphocyte |