International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 5 (2013) pp. 255-266
Evaluation of Anti-Arthritic, Antimicrobial and Amylase activities of Codium tomentosum from Andaman and Nicobar islands
Aparanji Poosarla1 *, Atika Raheem1 , Venu Gopal Sunkara1 , and Rajan P T2
1Department of Biochemistry, Andhra University, Visakhapatnam, India. 2Zoological Survey of India, Port Blair, Andaman and Nicobar Islands, India. *Corresponding author e-mail:
Codium tomentosum, a marine alga belongs to the family Codiaceae and order Bryopsidales, was collected at a depth of five meters from Pongi Balu area of Andaman Islands. Earlier, blood anticoagulant sulphated polysaccharides and genotoxicity/ antigenotoxicity, anti-oxidative capacities of C. tomentosum have been reported. The present investigation was carried out to determine the anti- arthritic, anti-bacterial, anti-fungal and amylase activities of C. tomentosum. The anti-arthritic activity was determined in C57/Black mice which were earlier elicited with the disease arthritis, treated using the ethanolic extract of the seaweed and anti-collagen antibody levels were estimated by ELISA. The anti-bacterial and antifungal activities were determined using the organic solvent extracts of C. tomentosum. The antibacterial activity was tested against both gram-positive and gram-negative bacteria namely Streptococcus species, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Proteus vulgaris. Moreover the antifungal activity was determined against the clinically important dermatophytes, Trichophyton mentagrophytes, Trichophyton rubrum and Aspergillus niger, Aspergillus flavus and Candida albicans. The amylase activity was determined using the aqueous extract. The C. tomentosum significantly decreased the antibody response in arthritic mice. In addition, the extracts of C. tomentosum were effective against both bacteria and fungi. The amylase activity was found to be stable at a temperature of 60°C and pH 8. This is the first report on anti-arthritic, antimicrobial and amylase activities of C. tomentosum. It showed potent inhibition against clinically important dermatophytes.
C57/ Black mice; ELISA; well diffusion method; SDS-PAGE; Dermatophytes