International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 223-230
Antimicrobial activity of extracellularly synthesized silver nanoparticles from marine derived actinomycetes
S.Deepa*, K. Kanimozhi and A.Panneerselvam
PG and Research Dept. of Botany and Microbiology, A.V.V.M Sri Pushpam College, (Autonomous), Poondi 613 503, Thanjavur, Tamilnadu, India *Corresponding author e-mail:
The process of development of reliable and eco-friendly metallic nanoparticles is an important step in the field of nanotechnology. To achieve this use of natural sources like biological systems becomes essential. In the present work, we have investigated extracellular biosynthesis of silver nanoparticles of AgNO3 using Thermoactinomyces sp. isolated from the marine sediment samples of Vellappallam Coast, Nagapattinam Dt, Tamil Nadu, India. Biosynthesized silver nanoparticles were confirmed by UV visible spectroscopy, the spectra showed a maximum absorption peak at 410 nm and the FT-IR analysis, provides evidence SEM confirmed the silver nanoparticles were spherical in shape and size in the range of 20-40 nm. The antimicrobial activities of silver nanoparticles were screened against common human pathogen (Bacillus subtilis, Enterobacter aerogenes, Enterococcus faecalis, Staphylococcus aureus and Streptococcus pyogenes) and five gram negative bacteria (Escherichia coli, Klebsiella oxytoca, K. pneumoniae, Salmonella typhi, and Vibrio cholerae). The results showed silver nanoparticles synthesized of AgNO3 displayed a significant antibacterial activity against Staphylococcus aureus and Bacillus subtilis.This study gives an innovative approach to develop new formulations based on metallic nanoparticles with antimicrobial properties to reach the pharmaceutical companies searching for new unconventional antibacterial agents.
Antibacterial activity; Marine sediment; silver nanoparticles; FT-IR; SEM.