International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 109-118

Cellulase from an estuarine Klebsiella ozeanae

V.Kalaiselvi* and S. Jayalakshmi

CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamilnadu, India *Corresponding author e-mail: micro_kalai@yahoo.co.in

ABSTRACT

To produce cellulase using solid state fermentation from Klebsiella Ozeanae cellulase producing bacterial population was isolated from water and sediment samples and plated on to cellulase agar medium. Among the 8 strains, Klebsiella ozeanae exhibited highest zone of lysis. Hence, it was selected for further production, optimization incubation period, temperature, pH, salt, nitrogen sources carbon source. The results showed that the optimum conditions for maximum celllulase production under SSF (Solid State Fermentation) by Klebsiella Ozeanae was found to be on the second day of incubation, optimization incubation period 48 h, temperature 35°C, pH 7, salt 3%, nitrogen sources peptone carbon source glucose.) and characterization of cellulase enzyme Fractional precipitation of proteins with varying saturation levels of ammonium sulfate was used as the first step in the separation and purification of cellulase enzyme from the mass culture of Klebsiella ozeanae grown in cellulose production broth and the cellulase enzyme molecular weight determination(32 kDa). Cellulase production by Estuarine Klebsiella ozeanae was significantly enhanced by optimization of medium composition and culture conditions. Given that cellulase is a natural product of bacteria in the marine environment, and that its presence enhances the bacterial use in ethanol production from cellulose

Keywords

Isolation; Sceening; Otimization; Purification; SDS PAGE; cellulase enzyme; Klebsiella ozeanae.