International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 8 (2013) pp. 73-78
Seroprevalence of leptospirosis in aborted dairy cattle in Peshawar district suburb, Khyber Pakhtunkhwa Pakistan
Khurshaid Anwar1 , Naveed Khan1* and Muhammad Mujtaba2 
1Research Officers, Veterinary Research Institute, Peshawar Pakistan 2Senior Research Officer, Veterinary Research Institute, Peshawar Pakistan *Corresponding author e-mail: 
Leptospirosis is a zoonosis of worldwide distribution, caused by Leptospira interrogans. It is a well known cause of bovine reproductive losses such as abortion, infertility, stillbirth and birth of weak calves. In this research, the relationship between the seroprevalence rate of Leptospira spp. infection and abortion in industrial dairy farms of Peshawar district Khyber Pakhtunkhwa Pakistan was studied. A total of 60 blood samples were taken from aborted cows and Sera were stored at -200C until analyzed. They were initially screened at serum dilution of 1:100 against six live antigens of Leptospira interrogans serovars Pomona, canicola, hardjo, ballum, icterohaemorrhagiae, grippotyphosa using microscopic agglutination test (MAT). The samples were considered positive if 50% of agglutination of leptospira in a dilution test serum of 1:100 were observed. Sera with positive results were titrated against reacting antigens in serial two-fold dilutions from 1:100 to 1:800. Out of total 60 samples antibodies were detected in 38 (63.33%) of the aborted cows, including 15 (39.47%) for Pomona followed by grippotyphosa 6 (15.78%), canicola, icterohaemorrhagiae and ballum 5 (each of which 13.15%) and 2 (5.26%) against L. hardjo.  
Leptospirosis; Abortion; Cattle; Micro- agglutination test; Peshawar; Seroprevalence.