International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 8 (2013) pp. 41-48
Characterization and antimicrobial activity of barley grain
(Hordeum vulgare) extract
Mohammad A. Jebor1 , Ali. Al-Saadi1 , Russul Hikmat Behjet2 , Mona Al-Terehi1 , Haider K.Zaidan1 , and Mohammed A.K.Al-Saadi3 
1University of Babylon, College of Science, 2University of Kufa -College of Science, 3University of Babylon College of Medicine, Hilla, Iraq *Corresponding author e-mail: 
The study aims to characterize and evaluate the antibacterial activity of Hordeum vulgare grain with modification using different methods, grain after and before modification was extracted by methanol-water methods then it characterized using Thinlayer chromatography, the phytochemical compound was detected by classical chemical methods. The antibacterial activity of plant extract was detected using three methods (well, disc and OD of broth culture).The results show that extract have 4 phytochemicals compounds before modification and one compound after modification. TLC profile results show that extract contained different polar compound that have different retardation factors. Antibacterial activity results show variations in bacterial sensitivity and different response by different methods. The OD of broth culture show the best results; five of the pathogenic bacteria inhibited by plant extract before modification, and two of these bacteria growth was decreased by plant extract after modification. 
Hordeum vulgare; OD of broth culture; Thin layer chromatography