International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 8 (2013) pp. 303-310
Biochemical changes in finger millet variety TNAU-914 inoculated with AM fungus Rhizophagus fasciculatus
Geeta B. Patil1 , H.C.Lakshman2 and A.Channabasava2*
1Department of Botany, P.C.Jobin Science Institute, Hubli, Karnataka, India 2Microbiology Laboratory, P.G. Department of Studies in Botany, Karnatak University, Pavate Nagar, Dharwad-580 003 *Corresponding author e-mail:
Rhizosphere is inhabited by variety of microorganisms, among, them mycorrhizal fungi are one of the predominate group of microorganisms. The present study was undertaken to understand the effect of mycorrhization on changes in passive physiology and biochemical substances associated with mycorrhizal roots of finger millet. Finger millet (Eleusine coracana Gaert.) is the third most important millet crop in India and it is highly nutritious with 0.33% calcium and 12.7% protein. Finger millet was grown with inoculation of AM fungus Rhizophagus fasciculatus. The visualization of fine structural components and chemical substances such as polysaccharides, total proteins, total lipids and different type enzymes within the mycorrhizal system and finger root tissue was analyzed. The results revealed that, greater amount of polysaccharides and proteins were observed in mycorrhizal roots compared to non-mycorrhizal once. Cytochrome oxidase enzyme was observed in both hyphae and vesicles but not in arbuscules. In contrast to this alkaline phosphatase, acid phosphatase and peroxidase were localized in both arbuscules and hyphae but not in vesicles. The total lipid was observed in both hyphae and vesicles. These results suggested that, further research is needed to explore activities associated with hidden wealth of soil.
Rhizophagus fasciculatus; Cytochrome oxidase; Eleusine coracana; poly- saccharides; Histochemistry