International Journal of Current Microbiology and Applied Sciences |
Effect of different concentration of sterilant and exposure time on sweet potato (Ipomoea batatas Lam) explants |
ISSN: 2319-7706 Volume 2 Number 8 (2013) pp. 14-20
|
N.C.Onwubiko*, C.S.Nkogho, C.P.Anyanwu, and G.C.Onyeishi |
Department of Crop Science and Technology, Federal University of Technology, Owerri, Nigeria *Corresponding author e-mail: onwubikouche@yahoo.com |
A B S T R A C T |
Investigation on the effect of different levels of concentration of sterilant (Sodium
hypochlorite or bleach) and exposure time on sweet potato explants was conducted
at Tissue Culture Laboratory of National Root Crop Research Institute Umudike, to determine the appropriate concentration of sterilant and exposure time to produce
clean culture. The experiment was a 5x5 factorial in a Completely Randomized Design (CRD) and the data collected were analyzed with Genstat Release VS 2008. Ipomea 87/00087 collected directly from the field was used in the study and was
incubated in a culture room at 28
oC 2 and exposed to artificial illumination of 2000 2500 lux for 16 hours daily for seven days. The result showed that Sodium
hypochlorite is not a very good sterilant as very high percentage of the explants was contaminated in all the experiments conducted. However a low percentage
(40%) of clean culture was observed at 20% sterilant concentration and 20 minutes exposure time. |
Keywords |
Sterilant (Sodium hypochlorite) concentration; exposure time, sweet potato explants. |