International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 7 (2013) pp. 217-232

Isolation and identification of Vibrios from marine food resources

K. Arunagiri1,2* , K. Jayashree2 and T. Sivakumar2

1Research and Development Centre, Bharathiar University, Coimbatore - 641 046, Tamil Nadu, India 2Department of Microbiology, Kanchi Shri Krishna College of Arts and Science, Kilambi, Kancheepuram, Tamil Nadu, India *Corresponding author e-mail: arunagirikumar01@gmail.com

A B S T R A C T

In the present study encompasses the incidence of Vibrio spp. in sea foods as a human pathogen, in contribute to the onset of sporadic and epidemic outbreaks of diarrhoeal disease in humans. The following aspects were covered during the present investigation. Isolation of Vibrio spp. in sea food samples collected from Kanchipuram and Walajapet local fish market. Antibiotic resistant pattern of isolated Vibrio spp. Hemolytic activity of randomly selected in Vibrio spp. High lights of the pattern investigations are, Vibrio spp. was readily recoverable in all the 21 samples tested viz., Finfishes and crustaceans. The entire biochemical test was carried out to identify the Vibrio spp. Antibiotic resistant pattern was observed in co-trimoxzole, amikacin, Gentamycin, Oxytetracycline were performed to find out antibiotic sensitivity pattern. A total of 21 strains were tested for hemolytic reaction 15 strains were positive for hemolytic production in blood agar plates and the remaining 6 strains were failed to lyses blood erythrocytes.

Keywords

Vibrio spp; sea food samples; Antibiotic resistant pattern; hemolytic reaction.