International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 7 (2013) pp. 116-122

Optimization of process parameters for the extracellular lipase production by newly isolated Pseudomonas aeruginosa KDP

K. Sujatha1 and K. Dhandayuthapani2 *

1Research and Development Centre, Bharathiar University, Coimbatore - 641 046, Tamil Nadu, India 2Department of Plant Biology and Biotechnology, Arignar Anna Govt. Arts College, Cheyyar 604 407, Tamil Nadu, India *Corresponding author e-mail: kdpani_bio@yahoo.co.in

A B S T R A C T

Optimization of process parameters for the extracellular lipases production by bacteria is most important because they have a broad range of biotechnological applications. In the present study lipase production, carried out in shake flasks with minimal medium and investigated the influence of different culture conditions including environmental (i.e., pH and Temperature) and nutritional (i.e., Ammonium nitrate, Urea, Selenium and Nickel) factors on the production of lipase by newly isolated Pseudomonas aeruginosa KDP. Maximum enzyme production was found to be at pH 7.0 and temperature 45 °C. The concentration 0.25mg/l of ammonium nitrate and urea present culture produced maximum lipase is 21.05 U/ml and 15.68 U/ml respectively. The maximum lipase production by P. aeruginosa KDP was found to be at 40µm/L Se and 60µm/L Ni supplemented culture. The correlation analyses of all the experiments proved that the physicochemical parameters and the lipase production are interdependent.

Keywords

Ammonium nitrate; Pseudomonas aeruginosa KDP; Lipase; Nicke; Selenium; Temperature; Urea; pH.