International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 6 (2013) pp. 64-69

Influence of media supplements on phenol biodegradation by Pseudomonas aeruginosa SPD10

Shweta1 and K. Dhandayuthapani2 *

1Research and Development centre, Bharathiar University, Coimbatore - 641 046, Tamil Nadu India 2PG Department of Plant Biology and Biotechnology,Arignar Anna Govt. Arts College, Cheyyar - 604 407, Tamil Nadu, India *Corresponding author e-mail: kdpani_bio@yhaoo.co.in

A B S T R A C T

The conventional methods of removing phenols from wastewater are very expensive. Degrading phenols and phenolic compounds by microorganisms is an alternative technology. A variety of microorganisms are known to utilize phenol as the sole carbon or energy source. Exploring the ability of microorganisms to metabolize phenols has received much attention due to the environmental persistence and its toxicity. In the time course study, Pseudomonas aeruginosa was able to completely utilize phenol within 3 days with 0.02 h 1 and 28.19 mg L 1 h 1 specific growth rate and substrate consumption rate, respectively. In the present study we also enhanced biodegradable capacity of P. aeruginosa by its media optimization. Phenol degradable media was supplemented with different organic nitrogen sources (Aspartic acid, Beef extract, Peptone, Tryptone and Yeast extract,) and metal ions (Copper, Iron, Nickel, Selenium, and Zinc). The maximum 89% of phenol degradation was found to be yeast extract and peptone present media and other nitrogen sources also showed the significant percent of biodegradation. The metal ions zinc and selenium contained media showed the maximum 87% of phenol degradation at a constant pH 7.0 and 35 oC. This work may be supported to select the best organic nitrogen source and metal ions for the large scale biodegradation of phenol.

Keywords

Bio- degradation; Metal ions; Nitrogen source; Phenol; Pseudomonas aeruginosa