International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 6 (2013) pp. 57-63

In vitro multiple shoot induction from nodal explants of Capsicum annum L. of kandhari variety

J. Philip Robinson* and M.Maheswari

Department of Biotechnology, K.S.Rangasamy College of Technology, Tiruchengode- 637 215, Tamil Nadu, India. *Corresponding author e-mail: philiprobin81@gmail.com

A B S T R A C T

An effective micropropagation system was developed through an enriched culture system for Capsicum annum L. of Kandhari variety, a traditional medicinal plant and nutritionally important spice crop. Proliferation of shoots were achieved on MS medium supplemented with 15 g l-1 sucrose, 8 g l-1 agar and fortified with benzyladenine (BAP)in the rage of 0.1 -1mg/l. A higher number of shoots were observed on an enriched MS medium supplemented with 0.5mg/l BAP. Then the shoots were subjected for root induction with the concentration of 1mg/ml. For the multiple shoot induction different combination of harmones were used. The well developed microshoots were transferred to rooting medium supplemented with IAA(1.0mg/l) for efficient rooting. The well developed shoots were transferred for hardening and the efficiency was 75% survival. This system can be successfully applied for mass propagation of Kandhari variety of capsicum annum.

Keywords

Multiplication; BAP; MS medium; rooting; accli- matization.