International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 6 (2013) pp. 299-306

An improved method for the in vitro propagation of Solanum melongena L.

J.Philip Robinson* and S.Saranya

Department of Biotechnology, K.S.Rangasamy college of Technology, Tiruchengode-637215, Tamil Nadu, India *Corresponding author e-mail: philiprobin81@gmail.com

A B S T R A C T

Brinjal is a tropical vegetable cultivated throughout the tropical regions of India. The varieties of Solanum melongena L. display a wide range of fruit shapes and colors. An experiment was conducted for the production of tolerant variety with the native variety of brinjal cv. Valuthalai produce long and white coloured fruits. The callus regeneration efficiency of tender shoot tip, hypocotyls, leaf and stem explant were analysed using MS basal media supplemented with different combinations and concentrations of phytohormones. The frequency of callus induction for shoot tip, hypocotyls, leaves, stem explant were 76.43%, 81.56%, 74.37% and 84.92% respectively and the maximum proliferation found at 0.6mg/l 2, 4-D. The multiple shoot induction was observed with the various concentrations of auxins and cytokinins such as BAP, KIN, IAA, NAA. The effectiveness was observed in their various combining rations for the induction and proliferation. The maximum efficiency of multiple shooting was obtained at 0.2mg/l BAP and 0.6mg/l NAA and o.4 mg/l IAA. The successful rooting was recorded on media supplemented with 0.4mg/l IBA.

Keywords

Shoot tips; co-cultivation; rooting, explants; phytohormones.