International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 6 (2013) pp. 280-292

Assessment of sperm DNA integrity by Toluidine blue staining technique in infertile patients and its relation to cryopreservation

S.Sasikumar*and D.Dakshayani

Nova IVI fertility centre, R.A. Puram, Chennai, Tamilnadu, India *Corresponding author e-mail: sasikumarsundararajan1@gmail.com

A B S T R A C T

Infertility id defined as inability to conceive after a year of regular unprotected intercourse. More than 30-40% of infertility cases are attributed to the male factor alone. Thirty semen samples were obtained at the fertility center of Billroth hospital for patients undergoing routine semen analysis for infertility. Then all the 30 samples were analyzed with toluidine blue (TB) to assess sperm DNA integrity. Out of 30 patients 6 have < than 20million/ml, 10 has < than 50% motility and the % of DNA damage is < 30% for 10 patients. If the count and motility was effected, there is a significant reduction of normal sperm parameters and the % of DNA damage is higher. Cryopreservation means storage at low temperature (196 C in liquid nitrogen), Cryopreservation of human spermatozoa is used extensively in artificial insemination and IVF programs. Freezing semen, has no effect on the of DNA denaturation. Cryopreservation is one important method to store the sperm for future use.

Keywords

Infertility; Male Factor; Toluidine blue; DNA damage; IVF programs.