| The aim of the present study is to investigate the anti-bacterial effect by using the leaf extract of Bauhinia species. Four species of Bauhinia viz..B.purpurea, B.galpini ,B.roxburghii, B.vahli among which anti bacterial effect of B.galpini, B.roxburghii were not reported ,were collected and washed thoroughly & dried. The leaf samples were extracted by the methanol , acetone , distil water as solvents . Those extracts were tested on 5 species of pathogenic bacteria viz.. Micrococcus luteus, Bacillus subtilis, Pseudomonas aeroginosa, Staphylococcus aureus and Klebsiella pleumonia by sterile filterpaper discs with methanol as solvent and MIC technique with acetone & Distil water as solvents. Different concentration of methanolic extract of Bauhinia leaf samples viz.. 50µg/ml, 100µg/ml, 200µg/ml, 400µg/ml were tested. Different zones of inhibition appears on agar plates and measured in millimetres. Comparatively, B. purpurea & B.galpini showed inhibition zones against Staphylococcus aureus and
Pseudomonas aeruginosa. B.galpini & B.roxburghii showed inhibition zones against Bacillus subtilis and Klebsiella pneumonia. Successfully, anti bacterial effect was determined in B.galpini & B. roxburghii ,which its antibacterial property
was not reported .The anti- bacterial activity of all the leaf extracts of 4 Bauhinia
species showed positive results even at least concentration of 50µg/ml.The effects of acetone & distil water extracts were Nill .The average results were obtained by Combination test. The methanolic extract of Bauhinia species were more effective than the standard anti biotic on most resistant bacteria viz.. Micrococcus luteus and Pseudomonas aeroginosa. |