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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 2942-2949
DOI: https://doi.org/10.20546/ijcmas.2018.709.366


Variability Analysis in Ginger (Zingiber officinale Rosc.) Somaclones Using RAPD Marker System
S.S. Gavande*, M.R. Shylaja and P.A. Nazeem
Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur - 680656, Kerala, India
*Corresponding author
Abstract:

Thirteen groups of somaclones (seven in Maran and six in Rio-de-Janeiro) were evaluated using Random Amplified Polymorphic DNA (RAPD). RAPD analysis using twelve selected primers produced 129 amplicons, 44 were polymorphic with an average of 3.66 polymorphic bands / primer and a polymorphism percentage of 34.10. The dendrograms generated based on RAPD profiles grouped the somaclones into two separate clusters. RAPD marker system showed that somaclones derived from cultivar Maran exhibited more variability than Rio-de-Janeiro. In group wise variability analysis using bulked DNA, the groups RC20 Gy and RSe10 Gy recorded higher variability from source parent cultivar. The variability exhibited in plant wise analysis using two selected primers was found very high (39%) as compared to group wise analysis (25%). The somaclone RC2Kr1031 of the callus regenerants and RSe1Kr1052 of the somatic embryo regenerants showed more variability exhibiting 59 and 53 per cent respectively from the source parent cultivar Rio-de-Janeiro.


Keywords: Bulked DNA, Ginger, RAPD, Somaclonal variation
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How to cite this article:

Gavande, S.S., M.R. Shylaja and Nazeem, P.A. 2018. Variability Analysis in Ginger (Zingiber officinale Rosc.) Somaclones Using RAPD Marker System.Int.J.Curr.Microbiol.App.Sci. 7(9): 2942-2949. doi: https://doi.org/10.20546/ijcmas.2018.709.366