Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 2857-2864
DOI: https://doi.org/10.20546/ijcmas.2018.709.355


Molecular Survey of Tobacco Leaf Curl Virus Disease in Different Parts of Karnataka
Gurudevi V. Navali* and K.T. Rangaswamy
Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bengaluru-560065, Karnataka, India
*Corresponding author
Abstract:

Surveys were conducted during 2016-18 revealed that the occurrence of the leaf curl disease on tobacco in major growing districts of Karnataka ranged from 19.60 to 31.01 during 2016-18. Among the six districts surveyed, highest per cent disease incidence of tobacco leaf curl virus was recorded in Shivamogga district (31.01) followed by Belagavi (25.59) and lowest per cent disease incidence of 19.60 of TbLCV was recorded in Chamarajangar district. The survey data imply that leaf curl disease is emerging as one of the major diseases of tobacco. For the detection of the virus, total DNA was extracted and PCR was carried out using pair of begomovirus specific Deng primers. All the isolates collected at different locations showed characteristic band of 550 bp whereas no amplicon was observed in healthy tobacco samples. This indicating the begomovirusis involved in causing the leaf curl disease in tobacco.


Keywords: Tobacco, Leaf curl disease, Survey, Detection
Download this article as Download

How to cite this article:

Gurudevi V. Navali and Rangaswamy, K.T. 2018. Molecular Survey of Tobacco Leaf Curl Virus Disease in Different Parts of Karnataka.Int.J.Curr.Microbiol.App.Sci. 7(9): 2857-2864. doi: https://doi.org/10.20546/ijcmas.2018.709.355