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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Online ISSN : 2319-7706
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Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 1990-1998
DOI: https://doi.org/10.20546/ijcmas.2018.709.241


Emergence of Virulent Pasteurella multocida and Mannheimia hemolytica in Sheep and Goats of Western Maharashtra, India
Sneha H. Daphal, Prashant P. Mhase, Mrunalini M. Pawade*, Prerana P. Shelke, Jyotica D. Sangle and Rahul P. Kolhe
Department of Microbiology, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Satara, Maharashtra, India
*Corresponding author
Abstract:

Pasteurella species are often encountered in small ruminants as major pathogens in respiratory illnesses resulting in heavy losses. Present study was aimed towards finding out the occurrence of Pasteurella spp. in small ruminants as respiratory pathogen and detection of virulence in them by phenotypic and genotypic characterization. In this study total 208 different samples like nasal swabs, lungs and heart blood were collected from diseased as well as healthy sheep (n=90) and goats (n=118) from the region of Western Maharashtra state of India. All the isolates (n=23) were processed for phenotypic characterization of Pasteurella spp. The isolates were confirmed with specific gene polymerase chain reaction by KMT and PHSSA gene PCR and CapA, CapD, and Lkt gene PCR for expression of virulence. Bacteriological examination of samples revealed that, out of 208 samples 23 (11.05%) were found culturally positive for presence of Pasteurella spp. Out of total Pasteurella spp. isolated, 16/23 (69.56%) were identified as P. multocida and 07/23 (30.43%) were identified as M. haemolytica. The isolates confirmed by Species specific KMT PCR for P. multocida resulted in cent percent positivity (100 %), while 57.12 percent of all phenotypically detected M. haemolytica were confirmed by PHSSA PCR. The virulence of P. multocida organisms was determined by expression of capsular antigen CapA found in 81.25% isolates and CapD gene found in 50.00 % isolates, while virulence in M. Haemolytica was determined by PHSSA and Lkt gene found in 57.12% and 42.85% isolates.


Keywords: Pasteurella multocida, Manheimia haemolytica, PCR, CAP A, CAP D, Sheep, PHSSA and LKT gene
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How to cite this article:

Sneha H. Daphal, Prashant P. Mhase, Mrunalini M. Pawade, Prerana P. Shelke, Jyotica D. Sangle and Rahul P. Kolhe. 2018. Emergence of Virulent Pasteurella multocida and Mannheimia hemolytica in Sheep and Goats of Western Maharashtra, India.Int.J.Curr.Microbiol.App.Sci. 7(9): 1990-1998. doi: https://doi.org/10.20546/ijcmas.2018.709.241