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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 1798-1803
DOI: https://doi.org/10.20546/ijcmas.2018.709.218


Bacterial Screening from Liquid Bile of Patients Underwent Laparoscopic Cholecystectomy Using Chromoginc Agar
Abdulameer Abdullah Al-Mussawi, Wisam Hamza Abbas and Zainab Alag Hassan
College of Nursing, University of Basra, Basra, Iraq
*Corresponding author
Abstract:

The main objective is to evaluate the incidence of bacterial pathogens in the liquid bile of patients underwent laparoscopic cholecystectomy using CHROMagar media. A Total of 34 samples were collected from patients suffering from cholecystolithiasis who were subjected to laparoscopic cholecystectomy by the same surgeon at Al-Sadr Teaching Hospital in Basra City for the period (31st of January 2016 to 30th of April 2016). It included 32 (94.1%) females and 2 (5.9%) males, aging between (23 – 61 years). Bile aspirates taken from each gallbladder in the operative room and directly inoculated onto brain heart infusion growth and incubated at 37°C for (24-48 hours). The isolates were identified by routine biochemical tests and CHROMagar media. A total of 34 samples were examined for detection of bacteria showed E. coil 11(32.4%), Enterococcus spp 7(20.6%), Pseudomonas aeruginosa 4(11.8%), Klebsiella pneumoniae 3(8.8%), Salmonella spp 3(8.8%) and Staphylococcus aureus 2(5.9%). A screening procedure using CHROMagar media gives better results and faster in identifying bacteria than conventional methods. Still the stasis secondary to gall stones is the commonest cause for cholecystitis and bacterial infection. Our work wishes to emphasize that E. coli presents at high frequency among other bacteria.


Keywords: Bacteria, Liquid Bile, Cholecystolithiasis, Cholecystectomy, CHROMagar media
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How to cite this article:

Abdulameer Abdullah Al-Mussawi, Wisam Hamza Abbas and Zainab Alag Hassan. 2018. Bacterial Screening from Liquid Bile of Patients Underwent Laparoscopic Cholecystectomy Using Chromoginc Agar.Int.J.Curr.Microbiol.App.Sci. 7(9): 1798-1803. doi: https://doi.org/10.20546/ijcmas.2018.709.218