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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 1566-1575
DOI: https://doi.org/10.20546/ijcmas.2018.709.188


Stability of Biosynthesised Silver Nanoparticles Using Achyranthes aspera Roots and Its Characterization
P.M. Smitha1*, Sharanagouda Hiregoudar1, Udaykumar Nidoni1, K.T. Ramappa1 and Sushilendra2
1Department of Processing and Food Engineering, College of Agricultural Engineering, University of Agricultural Sciences, Raichur- 584 101, Karnataka, India
2Department of Farm Machinery and Power Engineering, College of Agricultural Engineering, University of Agricultural Sciences, Raichur- 584 101, Karnataka, India
*Corresponding author
Abstract:

The present investigation was aimed to study the biosynthesis, stability and characterization of silver nanoparticles using Achyranthes aspera root extract. Synthesis of silver nanoparticles has been done by maintaining different AgNO3 concentrations (0.50, 1.00, 1.50 and 1.84 mM), temperature (25, 45, 75, 105 and 125 ºC) and pH conditions (4, 5, 7, 9 and 10). By analysing the data obtained during stability study, it was found that, combination of AgNO3 of 1.15 mM concentration, temperature at 45 ºC and pH of 9 was the best condition to synthesize the stable Ag NPs for one month. Characterization of synthesized silver nanoparticles was done by zetasizer, UV-Vis spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and atomic force microscopy (AFM). Particle size distribution of zetasizer indicated that the size of the biosynthesized silver nanoparticles was 23.21 nm and UV-Vis spectroscopy showed its absorbance peak at 420 nm, which confirmed the presence of Ag NPs. XRD analysis confirmed that, resultant Ag NPs were face-centered cubic in nature and AFM analysis showed surface area (103.97 µm2), selected particle height (0.12 µm) and width (1.10 µm). It was concluded that, green synthesis was an eco-friendly and most economical way to produce silver nanoparticles over the chemical and physical methods


Keywords: Biosynthesised Silver Nanoparticles, Achyranthes aspera, Roots
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How to cite this article:

Smitha, P.M., Sharanagouda Hiregoudar, Udaykumar Nidoni, K.T. Ramappa and Sushilendra. 2018. Stability of Biosynthesised Silver Nanoparticles Using Achyranthes aspera Roots and Its Characterization.Int.J.Curr.Microbiol.App.Sci. 7(9): 1566-1575. doi: https://doi.org/10.20546/ijcmas.2018.709.188