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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 1494-1503
DOI: https://doi.org/10.20546/ijcmas.2018.709.179


Evaluation of Pharmacokinetics, Antibacterial and Anti-Inflammatory Activities of Chrysin in Rat
Falguni Modi1*, S.K. Bhavsar2, J.H. Patel1, R.D. Varia1, L.C. Modi3 and Nitin Kale1
1Department of Veterinary Pharmacology and Toxicology
3Department of Veterinary Gynecology, College of Veterinary Sci. & A.H, Navsari Agricultural University, Navsari, Gujarat, India
2Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sci. & A.H, Anand Agricultural University, Anand, Gujarat, India
*Corresponding author
Abstract:

The pharmacokinetics, antibacterial and anti-inflammatory activities of Chrysin (100 mg/kg) were studied following intramuscular administration in rats. Drug concentration in rat plasma was determined using High Performance Liquid Chromatography (HPLC). The mean peak plasma drug concentration of 0.24 ± 0.01mg/mL was achieved at 0.25 h. The pharmacokinetic parameters like elimination half-life (t1/2β), apparent volume of distribution (Vdarea) and total body clearance of Chrysin were 0.52±0.03 h, 338.63±13.39 L/kg and 456.20±15.62 L/h/kg respectively were determined. In vitro and in vivo antibacterial activity of Chrysin was determined by microbroth dilution technique against different bacterial pathogens and in neutropenic rat intraperitoneal infection model, respectively. In the present study, Chrysin was found to have no in vitro antibacterial activity in range of 10 - 0.07 mg/mL. In in vivo bacterial colony count between test drug and positive drug (Chloramphenicol) indicated that Chrysin had no protective activity against Staphylococcus aureus in neutropenic rat intraperitoneal infection model. In the present study, Chrysin found to inhibit LPS induced nitric oxide production on RAW 264.7 macrophage cell line and COX-2 enzyme through ELISA method but significantly (p<0.01) lower to Meloxicam. In addition to this Chrysin (100 mg/kg) was found to be effective (34.67 ± 1.55 %) in carrageenan-induced paw edema assay in rat after 4h following intramuscular administration.


Keywords: Antibacterial, Anti-inflammatory, Pharmacokinetic, Chrysin, Rat
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How to cite this article:

Falguni Modi, S.K. Bhavsar, J.H. Patel, R.D. Varia, L.C. Modi and Nitin Kale. 2018. Evaluation of Pharmacokinetics, Antibacterial and Anti-Inflammatory Activities of Chrysin in Rat.Int.J.Curr.Microbiol.App.Sci. 7(9): 1494-1503. doi: https://doi.org/10.20546/ijcmas.2018.709.179