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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 1301-1311
DOI: https://doi.org/10.20546/ijcmas.2018.709.155


Mutations within the Quinolone Resistance Determining Region in Fluoroquinolone-Resistant Staphylococcus epidermidis Recovered from Different Ocular Isolates
Amrita Talukdar*, Kulandai Lily Therese and H. Nelofer Ali
L&T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu-600006, India
*Corresponding author
Abstract:

Staphylococcus epidermidis (S. epidermidis) is a common pathogen in ocular infection. Mutations contribute to drug resistance. We intended to identify mutations in genes within the quinolone resistance determining region (QRDR) of fluoroquinolone-resistant S. epidermidis ocular isolates and to study their phenotypic and genotypic correlation. A total of 50 phenotypically fluoroquinolones-resistant S. epidermidis isolates were studied. Fluroquinolones susceptibility was evaluated by Kirby- Bauer disk diffusion method. Polymerase chain reaction (PCR) was optimized and applied followed by DNA sequencing to detect mutations in gyrA, gyrB, parC and parE in the QRDR region among the fluoroquinolone-resistant S. epidermidis isolates recovered from ocular specimens. The majority of the samples (74%) were from conjunctival swabs (n = 37). gyrA, gyrB, parC, and parE genes were detected in 47 samples (94%). gyrA gene (n = 47) was the most common, followed by parE (n = 35), gyrB (n = 30) and parC (n = 28). In 25 isolates, all four mutated genes were present. In 25(50%) S. epidermidis isolates mutations were observed in all four genes of QRDR region of S. epidermidis genome. This is the first study in a tertiary eye care hospital in India to characterise ocular S. epidermidis for fluoroquinolone resistance which showed mutations were predominant in gyrA gene in the QRDR region compared to 3 other genes.


Keywords: Staphylococcus epidermidis (S. epidermidis), Fluoroquinolone-resistant
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How to cite this article:

Amrita Talukdar, Kulandai Lily Therese and Nelofer Ali, H. 2018. Mutations within the Quinolone Resistance Determining Region in Fluoroquinolone-Resistant Staphylococcus epidermidis Recovered from Different Ocular Isolates.Int.J.Curr.Microbiol.App.Sci. 7(9): 1301-1311. doi: https://doi.org/10.20546/ijcmas.2018.709.155