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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 1076-1084
DOI: https://doi.org/10.20546/ijcmas.2018.709.128


Minimization of Apoptosis like Changes Developed during Cryopreservation of Buffalo Bull Sperm by Supplementing Z-LEHD-FMK (Caspase Inhibitor)
Jasmer Dalal1*, Ajeet Kumar2, M. Honparkhe2, A.K. Singh2 and P.S. Brar2
1Department of Veterinary Gynaecology and Obstetrics, Lala Lajpat University of Veterinary and Animal University, Hisar-125004, Haryana, India
2Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India
*Corresponding author
Abstract:

Present study was undertaken to evaluate the anti-apoptotic effect of LEHD-FMK (caspase inhibitor) on buffalo bull sperm before and after semen freezing. Tris egg yolk extender was treated with Z-LEHD-FMK (0, 2, 4, 6, 10 and 20 µM) and used for extension of six aliquots of semen from each ejaculate to final concentration of 80 million sperm/ml. Sperm samples were analyzed for sperm motility, viability, plasma membrane integrity, mitochondrial membrane potential and PLA (Phospholipase) activity at pre-freeze and post thaw stages. There was no effect (P>0.05) of this caspase inhibitor on percent sperm motility, viability, plasma membrane integrity, active mitochondria and sperms with low PLA (Phospholipase) activity before semen freezing. However, Z-LEHD-FMK (2µM) treated sperm showed higher (P<0.05) motility with functionally intact membrane at post thaw stage as compared to control (0 µM). Percent post thaw viable sperm were higher (P<0.05) in Z-LEHD-FMK (2, 4 and 6 µM) treated semen sample as compared to control. Percent sperm with active mitochondria and low PLA activity were increased in dose dependent manner (P<0.05).


Keywords: Apoptosis like changes, Buffalo bull, Caspase inhibitor, Cryopreservation, Z-LEHD-FMK
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How to cite this article:

Jasmer Dalal, Ajeet Kumar, M. Honparkhe, A.K. Singh and Brar, P.S. 2018. Minimization of Apoptosis like Changes Developed during Cryopreservation of Buffalo Bull Sperm by Supplementing Z-LEHD-FMK (Caspase Inhibitor).Int.J.Curr.Microbiol.App.Sci. 7(9): 1076-1084. doi: https://doi.org/10.20546/ijcmas.2018.709.128