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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 930-939
DOI: https://doi.org/10.20546/ijcmas.2018.709.112


Isolation, Screening and Identification of Virulent Isolates of Bipolaris oryzae Causing Rice Brown Spot and Sarocladium oryzae Causing Sheath Rot Disease
G. Sobanbabu, K.G. Sabarinathan, V.K. Parthiban and V. Ramamoorthy*
Department of Plant Pathology, Agricultural College and Research Institute, (Tamil Nadu Agricultural University) Killikulam, Vallanadu, Thoothukudi (Dist), Tamil Nadu, India
*Corresponding author
Abstract:

Among the fungal diseases in rice plants, brown spot disease caused by (Bipolaris oryzae) and sheath rot disease caused by (Sarocladium oryzae) are considered as serious threats in rice cultivation in India. Rice plant samples showing the brown spot and sheath rot disease were collected from different places. Three different isolates of B. oryzae viz., B. oryzae isolate ASD1, B. oryzae isolate KKM1 and B. oryzae isolate CBE1were isolated from the infected rice leaves. Three different isolates of S. oryzae, viz., S. oryzae isolate KKM1, S. oryzae isolate KKM2 and S. oryzae isolate ASD1 were isolated from infected rice leaf sheath. Among the three B. oryzae isolates, B. oryzae isolate KKM1 grew vigorously and brought about the maximum disease incidence. Among the three S. oryzae isolates, S. oryzae isolate ASD1 grew vigorously and brought about the maximum disease incidence. S. oryzae was identified based on cultural and morphological characters which appeared as compact and showed constricted growth with fusiform, hyaline, smooth and single-celled conidia. The B. oryzae isolate KKM1 was confirmed as B. oryzae based on the morphological and molecular techniques by analyzing it’s ITS sequence. Thus, the present study shows the virulent isolates of both B. oryzae and S. oryzae could be used as potential isolates for further study on screening resistance rice cultivars.


Keywords: Bipolaris oryzae, Sarocladium oryzae, Molecular characterization, ITS, Phenotype analysis
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How to cite this article:

Sobanbabu, G., K.G. Sabarinathan, V.K. Parthiban and Ramamoorthy, V. 2018. Isolation, Screening and Identification of Virulent Isolates of Bipolaris oryzae Causing Rice Brown Spot and Sarocladium oryzae Causing Sheath Rot Disease.Int.J.Curr.Microbiol.App.Sci. 7(9): 930-939. doi: https://doi.org/10.20546/ijcmas.2018.709.112