National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Among the fungal diseases in rice plants, brown spot disease caused by (Bipolaris oryzae) and sheath rot disease caused by (Sarocladium oryzae) are considered as serious threats in rice cultivation in India. Rice plant samples showing the brown spot and sheath rot disease were collected from different places. Three different isolates of B. oryzae viz., B. oryzae isolate ASD1, B. oryzae isolate KKM1 and B. oryzae isolate CBE1were isolated from the infected rice leaves. Three different isolates of S. oryzae, viz., S. oryzae isolate KKM1, S. oryzae isolate KKM2 and S. oryzae isolate ASD1 were isolated from infected rice leaf sheath. Among the three B. oryzae isolates, B. oryzae isolate KKM1 grew vigorously and brought about the maximum disease incidence. Among the three S. oryzae isolates, S. oryzae isolate ASD1 grew vigorously and brought about the maximum disease incidence. S. oryzae was identified based on cultural and morphological characters which appeared as compact and showed constricted growth with fusiform, hyaline, smooth and single-celled conidia. The B. oryzae isolate KKM1 was confirmed as B. oryzae based on the morphological and molecular techniques by analyzing it’s ITS sequence. Thus, the present study shows the virulent isolates of both B. oryzae and S. oryzae could be used as potential isolates for further study on screening resistance rice cultivars.
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