Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 4636-4644
DOI: https://doi.org/10.20546/ijcmas.2018.708.489


Isolation of Aspergillus parasiticus and Detection of Aflatoxin B1 on Local Peanut (Arachis hypogaea L.) Varieties in Bali
A.S. Duniaji, W. Putu Arisandhi, N.N. Puspawati and C.D. Ni Putu Novilia
Department of Food Science and Technology University of Udayana Bali, Indonesia
*Corresponding author
Abstract:

This study was aimed to determine the contamination of Aspergillus parasiticus and aflatoxin B1 on Local varieties of peanuts in Bali. A total of 18 Local varieties of peanut were taken at several farmers in the field on each district in Bali. This study used descriptive analysis that showed in the form of figure and table. Samples were isolated using PDA (Potato Dextro Agar) during the incubation period 5 days (120 hours) at room temperature (29 °C) and further purified by the media DRBC (Dichloran Rose Bengal Chlorampenicol) and Aflatoxin B1 was analyzed using the Enzyme Linked Immunosorbent Assay (ELISA) [Chinaphuti, 2003]. The results showed A. parasiticus contaminated Local varieties of peanuts are the lowest in Bali on the sample Amlapura Local 1, Gianyar Local 1, Mangupura Local 1 and Tabanan Local 1 were 1.0 x 104 cfu ml-1, and the highest population present in the sample Singaraja Local 1 was 1.0 x 105 cfu ml-1. Aflatoksin B1 content of the peanut contaminating Local varieties ranged from 2.4 ± 0.57 ppb – 100 ppb. Local varieties of peanuts for consumption according to Codex standards maximal 15 ppb is 94.4 % (ranged from 0-15 ppb)


Keywords: Local peanuts, A. parasiticus, Aflatoxin B1
Download this article as Download

How to cite this article:

Duniaji, A.S., W. Putu Arisandhi, N.N. Puspawati and Ni Putu Novilia, C.D. 2018. Isolation of Aspergillus parasiticus and Detection of Aflatoxin B1 on Local Peanut (Arachis hypogaea L.) Varieties in Bali.Int.J.Curr.Microbiol.App.Sci. 7(8): 4636-4644. doi: https://doi.org/10.20546/ijcmas.2018.708.489