National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Trichinellosis is important food-borne parasitic zoonoses caused by consumption of raw or under-cooked meat from a wide variety of wild and domestic mammals. Pork is consumed across the various pockets of India and it is most important source of infection to humans for Trichinellosis. Recent reports on presence of Trichinella spp. in pork sold in Maharashtra, India is concern for consumers. Therefore the study was planned to check occurrence of Trichinella in pork sold in Mumbai by Acid-pepsin digestion assay and multiplex PCR. Acid-pepsin digestion assay could not able to isolate single larvae from 161 samples similar results were also observed by standardized multiplex PCR. Though none of the sample was found to be positive for Trichinella spp. in present study but standardized multiplex PCR assay using standard larvae of T. spiralis and T. britovi can be useful for differentiation of T. britovi and T. spiralis larvae in Indian condition. Regular monitoring and surveillance of trichinellosis in pigs and other reservoirs by acid pepsin digestion assay and multiplex PCR is necessary.
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