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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 4451-4457
DOI: https://doi.org/10.20546/ijcmas.2018.708.470


Studies on Occurrence of Trichinellosis in Pigs and Its Molecular Characterization Using Multiplex PCR in Maharashtra, India
L.N. Kale1, R.N. Waghamare2*, V.M. Vaidya1, R.J. Zende1, A.M. Paturkar1, R.G. Shende1, N.B. Aswar1 and D.P. Kshirsagar1
1Department of Veterinary Public Health, Bombay Veterinary College, Mumbai, India
2Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Parbhani, 431402, India
*Corresponding author
Abstract:

Trichinellosis is important food-borne parasitic zoonoses caused by consumption of raw or under-cooked meat from a wide variety of wild and domestic mammals. Pork is consumed across the various pockets of India and it is most important source of infection to humans for Trichinellosis. Recent reports on presence of Trichinella spp. in pork sold in Maharashtra, India is concern for consumers. Therefore the study was planned to check occurrence of Trichinella in pork sold in Mumbai by Acid-pepsin digestion assay and multiplex PCR. Acid-pepsin digestion assay could not able to isolate single larvae from 161 samples similar results were also observed by standardized multiplex PCR. Though none of the sample was found to be positive for Trichinella spp. in present study but standardized multiplex PCR assay using standard larvae of T. spiralis and T. britovi can be useful for differentiation of T. britovi and T. spiralis larvae in Indian condition. Regular monitoring and surveillance of trichinellosis in pigs and other reservoirs by acid pepsin digestion assay and multiplex PCR is necessary.


Keywords: Prevalence, Multiplex PCR, Acid-pepsin digestion, Trichinella
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How to cite this article:

Kale, L.N., R.N. Waghamare, V.M. Vaidya, R.J. Zende, A.M. Paturkar, R.G. Shende, N.B. Aswar and Kshirsagar, D.P. 2018. Studies on Occurrence of Trichinellosis in Pigs and Its Molecular Characterization Using Multiplex PCR in Maharashtra, India.Int.J.Curr.Microbiol.App.Sci. 7(8): 4451-4457. doi: https://doi.org/10.20546/ijcmas.2018.708.470