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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(8): 4364-4372
DOI: https://doi.org/10.20546/ijcmas.2018.708.458


A Preliminary in vitro Study to Evaluate Poly-3-Hydroxy Butyrate as an Anticoccidial Agent against Oocysts of E. tenella
T. Aadithya1, S. Meignanalakshmi1*, M. Raman2, M. Parthiban1 and K. Vijayarani1
1Department of Animal Biotechnology, Madras Veterinary College, TANUVAS,Chennai-7, India
2Translational Research Platform for Veterinary Biologicals, MadhavaramMilk Colony, TANUVAS, Chennai-51, India
*Corresponding author
Abstract:

The present study was conducted to evaluate the effect of Poly-3-Hydroxy butyrate against the sporulation of oocyst of E. tenella. In this study four different doses of PHB which was extracted from the Bacillus subtilis culture, 10, 20, 50and 100mg were used with 2.5% potassium dichromate as positive control and distilled water as negative control. The experiment was carried out in 24 well plates. The sporulation efficiency was evaluated by counting the sporulated and unsporulated oocysts using haemocytometer and percentage efficiency of sporulation inhibition was calculated. Data were analysed using chi square test. The PHB at 100mg concentration showed significant effect on sporulation inhibition when compared to other doses of PHB and positive control.


Keywords: Coccidiosis, Sporulation inhibition, TEM and SEM analysis, E. tenella oocyst, PHB
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How to cite this article:

Aadithya, T., S. Meignanalakshmi, M. Raman, M. Parthiban and Vijayarani, K. 2018. A Preliminary in vitro Study to Evaluate Poly-3-Hydroxy Butyrate as Anticoccidial Agent against Oocysts of E. tenella.Int.J.Curr.Microbiol.App.Sci. 7(8): 4364-4372. doi: https://doi.org/10.20546/ijcmas.2018.708.458